Prediction of β-lactamase and its class by Chou’s pseudo-amino acid composition and support vector machine. β-Lactam class of antibiotics is used as major therapeutic agent against a number of pathogenic microbes. The widespread and indiscriminate use of antibiotics to treat bacterial infection has prompted evolution of several evading mechanisms from the lethal effect of antibiotics. β-Lactamases are endogenously produced enzyme that makes bacteria resistant against β-lactam antibiotics by cleaving the β-lactam ring. On the basis of primary structures, β-lactamase family of enzymes is divided into four classes namely A, B, C and D. Class B are metallo-enzymes while A, C and D does not need any metal in the enzyme catalysis. In the present study we developed a SVM based two level β-lactamases protein prediction method, which differentiate β-lactamases from non-β-lactamases at first level and then classify predicted β-lactamases into different classes at second level. We evaluated performance of different input vectors namely simple amino acid composition, Type-1 and Type-2 Chou’s pseudo amino acid compositions. Comparative performances indicated that SVM model trained on Type-1 pseudo amino acid composition has the best performance. At first level we were able to classify β-lactamases from non-β-lactamases with 90.63% accuracy. At second level we found maximum accuracy of 61.82%, 89.09%, 70.91% and 70.91% of class A, class B, class C and class D, respectively. A web-server as well as standalone, PredLactamase, is also developed to make the method available to the scientific community, which can be accessed at http://188.8.131.52/mkumar/predlactamase.
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References in zbMATH (referenced in 5 articles )
Showing results 1 to 5 of 5.
- Jia, Jianhua; Liu, Zi; Xiao, Xuan; Liu, Bingxiang; Chou, Kuo-Chen: pSuc-Lys: predict lysine succinylation sites in proteins with PseAAC and ensemble random forest approach (2016)
- Jiao, Ya-Sen; Du, Pu-Feng: Predicting Golgi-resident protein types using pseudo amino acid compositions: approaches with positional specific physicochemical properties (2016)
- Yang, Lianping; Zhang, Xiangde; Fu, Haoyue; Yang, Chenhui: An estimator for local analysis of genome based on the minimal absent word (2016)
- Ju, Zhe; Cao, Jun-Zhe; Gu, Hong: iLM-2L: a two-level predictor for identifying protein lysine methylation sites and their methylation degrees by incorporating K-gap amino acid pairs into Chou’s general PseAAC (2015)
- Kumar, Ravindra; Srivastava, Abhishikha; Kumari, Bandana; Kumar, Manish: Prediction of $\beta$-lactamase and its class by Chou’s pseudo-amino acid composition and support vector machine (2015)