3D reconstruction from electron microscope images with TxBR. Tomographic reconstruction from large format electron microscope requires special procedures to handle geometric distortions arising from electron optics as opposed to light-ray optics. In particular, electrons travel in curvilinear paths through the sample and defocus and other aberrations can be more severe. We report on a software package, TxBR, which solves the inverse problem associated with a generalized ray transform. We also review the prospects for automated alignment, and discuss possible approaches.

This software is also peer reviewed by journal TOMS.

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