NEBcutter: a program to cleave DNA with restriction enzymes. NEBcutter, version 1.0, is a program available via a web server ( that will accept an input DNA sequence and produce a comprehensive report of the restriction enzymes that will cleave the sequence. It produces a variety of outputs including restriction enzyme maps, theoretical digests and links into the restriction enzyme database, REBASE ( Importantly, its table of recognition sites is updated daily from REBASE and it marks all sites that are potentially affected by DNA methylation (Dam, Dcm, etc.). Many options exist to choose the enzymes used for digestion, including all known specificities, subsets of those that are commercially available or sets of enzymes that produce compatible termini.

References in zbMATH (referenced in 3 articles )

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  1. Montes, Pablo; Memelli, Heraldo; Ward, Charles B.; Kim, Joondong; Mitchell, Joseph S. B.; Skiena, Steven: Optimizing restriction site placement for synthetic genomes (2012)
  2. Li, Chao; Li, Yuhua; Zhang, Xiangmin; Stafford, Phillip; Dinu, Valentin: Icrpfinder: a fast pattern design algorithm for coding sequences and its application in finding potential restriction enzyme recognition sites (2009) ioport
  3. Vincze, Tamas; Posfai, Janos; Roberts, Richard J.: Nebcutter: a program to cleave DNA with restriction enzymes. (2003) ioport