PESTO - Parameter EStimation TOolbox: PESTO is a widely applicable and highly customizable toolbox for parameter estimation in MathWorks MATLAB. It offers state-of-the art algorithms for optimization and uncertainty analysis, which work in a very generic manner, treating the objective function as a black box. Hence, PESTO can be used for any parameter estimation problem, which provides an objective function in MATLAB. PESTO has been used in various computational biology research projects. PESTO features include: Multistart optimization; Sampling routines; Profile-likelihood analysis; Visualization routines and more