RuleBender: A visual interface for rule-based modeling. Results: We introduce RuleBender, a novel visualization system for the integrated visualization, modeling and simulation of rule-based intracellular biochemistry. We present the user requirements, visual paradigms, algorithms and design decisions behind RuleBender, with emphasis on visual global/local model exploration and integrated execution of simulations. The support of RBM creation, debugging, and interactive visualization expedites the RBM learning process and reduces model construction time; while built-in model simulation and results with multiple linked views streamline the execution and analysis of newly created models and generated networks. Conclusion: RuleBender has been adopted as both an educational and a research tool and is available as a free open source tool at A development cycle that includes close interaction with expert users allows RuleBender to better serve the needs of the systems biology community.