cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination. CryoSPARC combines powerful innovations in 3D reconstruction algorithms with specially designed software to provide a streamlined end-to-end single particle cryo-EM workflow. Rapidly solve high-resolution structures of biologically important targets, with advanced tools for membrane proteins, heterogeneous samples, and flexible molecules. Process 3D refinements in minutes on GPU.